| Name | Date | Defenses |
| Kelly Pavlik | September 29, 2007 | 1 |
| Jermain Taylor | July 16, 2005 | 3 |
| Bernard Hopkins | September 18, 2004 | 1 |
| Oscar De La Hoya | June 5, 2004 | 0 |
| Felix Sturm | September 13, 2003 | 1 |
| Hector Javier Velazco
def. Andras Galfi | May 10, 2003 | 0 |
| Title vacated | 2002 | 0 |
| Harry Simon | April 6, 2002 | 0 |
| Armand Krajnc | November 3, 2001 | 0 |
| Krajnc vacated the title on June 4, 2001, while in a dispute with Universum Box-Promotion, however the WBO gave Krajnc back the title. | | 0 |
| Harry Simon – Interim
def. Hacine Cherifi | July 21, 2001 | 0 |
| Armand Krajnc | November 27, 1999 | 2 |
| Jason Matthews
def. Ryan Rhodes | July 17, 1999 | 0 |
| Title vacated | 1999 | 0 |
| Bert Schenk
def. Freeman Barr | January 30, 1999 | 1 |
| Title vacated | 1998 | 0 |
| Otis Grant
def. Ryan Rhodes | December 13, 1997 | 1 |
| Title vacated | 1997 | 0 |
| Lonnie Bradley
def. David Mendez | May 19, 1995 | 6 |
| Title vacated | 1994–1995 | 0 |
| Steve Collins | May 11, 1994 | 0 |
| Chris Pyatt
def. Sumbu Kalambay | May 19, 1993 | 2 |
| Title vacated | 1991 | 0 |
| Gerald McClellan
def. John Mugabi | November 20, 1991 | 0 |
| Title vacated | 1991 | 0 |
| Chris Eubank | November 18, 1990 | 3 |
| Nigel Benn | April 29, 1990 | 1 |
| Doug DeWitt
def. Robbie Sims | April 18, 1989 | 1 |
